Journal: BioMed Research International

Article Title: Autophagy and Human Parturition: Evaluation of LC3 Expression in Placenta from Spontaneous or Medically Induced Onset of Labor

doi: 10.1155/2013/689768

Figure Lengend Snippet: Clinical characteristics and autophagy.

Article Snippet: After washing, the blots were incubated with antirabbit horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, dilution 1 : 10000). α -Tubulin was used for data normalization ( α -tubulin (E-19)-R antibody, Santa Cruz Biotechnology, dilution 1 : 1000).

Techniques:

Journal: BioMed Research International

Article Title: Autophagy and Human Parturition: Evaluation of LC3 Expression in Placenta from Spontaneous or Medically Induced Onset of Labor

doi: 10.1155/2013/689768

Figure Lengend Snippet: LC3, CRF, and HIF-1 α expression. (a) for each antibody the spatial distribution of immunohistochemical staining was the same in cases with spontaneous and induced labor. (b) The analysis of data from optical density values of Western blotting bands shows no differences of LC3-II and HIF-1 α expression between groups. On the contrary CRF is higher in spontaneous labor. LC3-II, HIF-1 α , and CRF are normalized onto α -tubulin. (c) No correlation between LC3-II and CRF expression was found. SP: spontaneous labor; PG: induction with prostaglandin; PO: induction with prostaglandin and oxytocin; OX: induction with oxytocin.

Article Snippet: After washing, the blots were incubated with antirabbit horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, dilution 1 : 10000). α -Tubulin was used for data normalization ( α -tubulin (E-19)-R antibody, Santa Cruz Biotechnology, dilution 1 : 1000).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: BioMed Research International

Article Title: Autophagy and Human Parturition: Evaluation of LC3 Expression in Placenta from Spontaneous or Medically Induced Onset of Labor

doi: 10.1155/2013/689768

Figure Lengend Snippet: Autophagy and clinical correlation. (a) A significant correlation was found between LC3-II expression and prepregnancy maternal body mass index. Open circles: spontaneous onset of labor; closed circles: pharmacological induction of labor. LC3-II = 3.6–0.11 BMI. R 2 0.29. P = 0.003. (b) LC3 dots formation identified by immunofluorescence in two cases of our population. Lower panel: PG group, patient with BMI = 16.6 kg/m 2 , LC3-II/ α tubulin = 2.07, and umbilical cord pH = 7.22. Upper panel: PG group, patient with BMI = 26.6 kg/m 2 , LC3-II/ α tubulin = 1.0, and umbilical cord pH = 7.25. (c) No relationship was found between maternal pre-pregnancy BMI and pH in umbilical cord. Open circles: spontaneous onset of labor; closed circles: pharmacological induction of labor. (d) No relationship was found between autophagy and pH in umbilical cord. Open circles: spontaneous onset of labor; closed circles: pharmacological induction of labor. (e) No relationship was found between autophagy and pO 2 (mmHg) in umbilical cord. Open circles: spontaneous onset of labor; closed circles: pharmacological induction of labor. BMI: body mass index.

Article Snippet: After washing, the blots were incubated with antirabbit horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, dilution 1 : 10000). α -Tubulin was used for data normalization ( α -tubulin (E-19)-R antibody, Santa Cruz Biotechnology, dilution 1 : 1000).

Techniques: Expressing, Immunofluorescence